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functional remodeling of human benign prostatic tissue in vivo by spontaneously Inm

www.crio-cord.com - regeneración de los tejidos adultos o de remodelación, se sugiere iniciar de madre multipotentes, las células progenitoras and stem cells. We have recently reported two new human adults tumorigenic epithelial cell lines and prostate NHPrE1 BHPrE1, which have been generated and characterized in our laboratory. NHPrE1 cells were designated as putative stem cells, showing high levels of expression of stem cells of proteins associated with CD133, CD44, Oct4 and PTEN detected by immunofluorescence (IF) tincióny Western blot. BHPrE1 is defined as intermediate or transit amplifying (TA) cells that express the cell cycle regulation, biomarkers related to p63, p53, p21/WAF1 and RB. We used a tissue recombination xenograft mouse model to compare functional regeneration of human tissues from the prostate gland in vivo.

A series of recombinant tissue by different proportions of the mixture of cells with prostatic NHPrE1 or inductive BHPrE1 rat urogenital sinus mesenchyme (UGM), were transplanted to the kidney capsule of immunodeficient mice SCID male. Both lines BHPrE1 NHPrE1 cell and were able to regenerate human model, the acinar architecture benign secretory ducts in vivo, containing intact basal and luminal epithelial layers. Cytokeratin appropriate profiles were observed in epithelial tissue layers. The differentiation of prostate-associated proteins, such as the androgen receptor (AR), prostate specific antigen (PSA), NKX3.1 and 15-lipoxygenase-2 (15 - LOX-2), were properly expressed in epithelial remodeling.

NHPrE1 Only 10 cells were able to regenerate structure of benign prostatic glandular when recombined with rat UGM, while 200,000 BHPrE1 cells were necessary to achieve the same. These results suggest a higher proportion of progenitor cells / stem cells NHPrE1 cells than in cells BHPrE1. The data presented supports the idea that in vivo functional remodeling of human benign prostatic ductal acinar architecture is more efficient when initiated from progenitor course rather than intermediate or transit amplifying cé cells.

NHPrE1 cell lines and BHPrE1 share some important characteristics that make them relevant to the research community in urology. First, different molecular characteristics are indicative of possible mechanisms underlying their different abilities, probably due to their different degrees of "stemness."

also of great importance to the field of prostate research, these cells retain expression of biomarkers key human prostate, including the AR and the ability to express differentiation markers associated with PSA, NKX3.1 and 15-LOX-2 when functionally reconstructed in a tissue recombination model while xenotransplantation , n. This represents a breakthrough in this area compared to most current lines of prostate cells. The ability to regenerate functionally benign human prostate ductal acinar architecture with luminal and basal epithelial sub populations that express the appropriate cytokeratin and prostate-related biomarkers with no differentiation of cell lines of prostate currently available to the research community. Both cell lines

BHPrE1 NHPrE1 and provide important data on phenotypes of progenitor cells and medium and represent significant new tools for the elucidation of the molecular mechanisms of human prostate regeneration and pathogenesis carcinogenesis. And functionally regenerated recombinant benign prostate tissue, these cell lines will useful models for investigating the biological and molecular mechanisms of the cell related to the genesis of benign and malignant human prostate.