www.crio-cord.com - adult tissue regeneration or remodeling, we suggest start of multipotent stem and progenitor cells \u0026lt;a href = " http://www.crio -cord.com / "> stem \u0026lt;/ a>. We have recently reported two new human adults tumorigenic lines of prostate epithelial cells and BHPrE1 NHPrE1 that have been generated and characterized in our laboratory. NHPrE1 cells were designated as putative stem cells, showing high levels of expression of stem cells of proteins associated with CD133, CD44, Oct4 and PTEN detected by immunofluorescence (IF) tincióny Western blot. BHPrE1 is defined as intermediate or transit amplifying (TA) cells that express the cell cycle regulation, biomarkers related to p63, p53, p21/WAF1 and RB. We used a recombination tissue xenotransplantation mouse model to compare functional regeneration of human tissues from the prostate gland in vivo.
A series of recombinant tissue by different proportions of the mixture of cells with prostatic NHPrE1 or inductive BHPrE1 rat urogenital sinus mesenchyme (UGM), were transplanted to the kidney capsule of immunodeficient mice SCID male. Both cell lines could BHPrE1 NHPrE1 and regenerate the human model, the acinar architecture benign secretory ducts in vivo, containing intact basal and luminal epithelial layers. Cytokeratin appropriate profiles were observed in epithelial tissue layers. The differentiation of prostate-associated proteins, such as the androgen receptor (AR), prostate specific antigen (PSA), NKX3.1 and 15-lipoxygenase-2 (15 - LOX-2), were properly expressed in epithelial remodeling.
NHPrE1 Only 10 cells were able to regenerate the structure of benign prostatic glandular when recombined with rat UGM, while 200,000 BHPrE1 cells were necessary to achieve the same. These results suggest a greater proportion of progenitor cells / \u0026lt;a href=" "> http://www.crio-cord.com/ stem cells \u0026lt;/ a> NHPrE1 cells in the BHPrE1 cells. The data presented supports the idea that in vivo functional remodeling of human benign prostatic ductal acinar architecture is more efficient when initiated from progenitor course rather than intermediate or transit amplifying cé cells.
cell lines BHPrE1 NHPrE1 and share a number of features make major interest to the research community in urology. First, different molecular characteristics are indicative of possible mechanisms underlying their different abilities, probably due to their different degrees of "stemness."
also of great importance to the field of prostate research, these cells retain expression of key biological markers of human prostate, including AR and the ability to express the differentiation markers associated with PSA, NKX3.1 and 15-LOX-2 when functionally reconstructed in a tissue recombination xenograft mouse model. This represents a breakthrough in this area compared to most current lines of prostate cells. The ability to regenerate functionally benign human prostate ductal acinar architecture with luminal and basal epithelial sub populations that express the appropriate cytokeratin and prostate cancer biomarkers associated with no differentiation of cell lines Prostate currently available to the research community. Both cell lines
BHPrE1 NHPrE1 and provide important data on phenotypes of progenitor cells and medium and represent significant new tools for the elucidation of the molecular mechanisms of human prostate regeneration and pathogenesis carcinogenesis. And functionally regenerated recombinant benign prostate tissue, these cell lines are useful models for investigating the biological mechanisms and molecular cell related to the genesis of benign and malignant human prostate.
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